ISSN: 2822-0838 Online

GC/MS Based Metabolite Profiling and Biological Activity of Leaves and Flower Heads of Tanacetum Cinerariifolium (Trevir.) Sch.Bip.

Milena Nikolova *, Vladimir Ilinkin, Elina Yankova-Tsvetkova, Marina Stanilova and Strahil Berkov
Published Date : 2022-07-11
DOI : https://doi.org/10.12982/CMUJNS.2022.038
Journal Issues : Number 3, July-September 2022

Abstract  Flower heads and leaves of Tanacetum cinerariifolium (Trevir.) SchBipwere examined separately for bioactive compounds and biological activityThe compounds were identified by GC/MS analysisStudied extracts were evaluated for free radical scavenging activity, and for inhibition of acetylcholinesterase and seed germinationFree phenolic acids and flavonoid aglycones were found in larger quantities in the leaves than in the flower heads, where sterols, fatty acids, pyrethrins, sugars, sugar derivatives and bound phenolic acids predominatedSignificant antiradical activity (IC50 /mLand low acetylcholinesterase inhibitory activity (IC50 >1 mg/mLwas foundModerate inhibitory activity against germination and root elongation of Lolium perenne Lseeds was determinedThe study presents for the first time detailed data on the content of phenolic acids in free, esterified and insoluble-bound forms in this speciesSome of the identified flavonoids from the leaf extract are reported for the first time in the species.

 

KeywordsGC/MS, Flavonoids, Phenolic acids, Asteraceae (Composite)

 

Funding: This research was supported by the Bulgarian National Science Fund, Bulgarian Ministry of Education and Science (Grant DN 16/2, 11.12.2017).

 

Citation: Nikolova, M., Ilinkin, V., Yankova-Tsvetkova, E., Stanilova, Mand Berkov, S2022GC/MS based metabolite profiling and biological activity of leaves and flower heads of Tanacetum cinerariifolium (Trevir.) Sch.Bip.. CMU JNatSci21(3): e2022038.

 

INTRODUCTION

Tanacetum cinerariifolium (Trevir.) Sch. Bip. (dalmatian pyrethrum) syn.: Chrysanthemum cinerariifolium (Trevis.) Vis., Pyrethrum cinerariifolium Trevis. is herbaceous perennial herb, belonging to the Asteraceae (Composite) family. The plant forms rosette of many complex 5-6 lobed deeply incised fluffy leaves covered with hairs. The capitula consist of a large number of yellow tubular florets surrounded on their periphery by a row of ray linguals florets with long white petals. Tcinerariifolium is well known for its insecticidal properties [Grdiša et al., 2009], which are determined by the pyrethrins contained in all organs of the plant, particularly in the flower heads which contain more than 90% of the total pyrethrin content of the plant [Jovetic et al., 1994]. The species occurs naturally in a limited area along the east Adriatic coast [Nikolić et al., 2007; Grdiša et al., 2014], but it is cultivated as an agricultural crop in different countries around the world as Australia [Fulton, 1998], Uganda [Greenhill, 2007], France, Chile [Grdiša et al., 2009], Kenya, Tanzania, Rwanda and Ecuador [Casida, 1980] Austria, Colombia, Cyprus, Germany, Hungary, Italy, Jawa, Korea, Nepal, Pakistan, Peru, Spain, Sri Lanka, Switzerland, Ukraine, Vietnam, West Himalaya and other https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:252275-1. Research on the content of secondary metabolites by the species has mainly been focused on studies related to Pyrethrin-I, Cinerin-I, Jasmolin-I, Pyrethrin-II, Cinerin-II, Jasmolin-II and their variation in naturally occurring populations or agricultural crops [Grdiša et al., 2009; Fulton, 1998; Kiriamiti  et al, 2003; Grdiša et al., 2013]. Although less, there are reports on the content of other bioactive compounds in the species such as sesquiterpene lactones, triglycerides, fatty acids, terpenoids, carotenoids, chrysanthin, inulin [Maciver, 1995; Pîrvu et al., 2008; Ramirez et al., 2013]. The most studied and in detail biological activity of the species is insecticidal [Schoenig 1995; Crosby et al. 1995; Greenhill 2007; Cai et al., 2010] but a few studies reveal the presence of antiviral and antimicrobial activity for extracts of Tcinerariifolium [Marongiu et al., 2009]. Due to the lower content of pyrethrins in the leaves, they are not used in the extraction of natural pyrethrins and they have been insufficiently studied. The phytochemical profile of flower heads is also important because after the extraction of pyrethrins with a non-polar solvent, there are other more polar substances that would have the potential to find application for other areas. All the data presented so far determine the purpose of the study – comparative GC/MS based analysis of the metabolite profiles of extracts from flower heads and leaves of Tcinerariifolium. Also the extracts obtained were tested for free radical scavenging activity and for inhibition of seed germination and acetylcholinesterase.  

 

MATERIALS AND METHODS

Materials

Flower heads and leaves of Tcinerariifolium plants were collected during the flowering stage from the ex situ collection of the Institute of Biodiversity and Ecosystem Research, Sofia, Bulgaria (Figure.1).

 

Extraction of plant material

Acetone exudates were prepared from air-dried, not ground (flower heads or leaves, per 1 g) by rinsing with acetone for 5 min to dissolve the lipophilic compounds accumulated on the surfaces. Methanolic extracts (5 g) were prepared from air-dried, ground flower heads or leaves, by classical maceration with methanol for 24 h.

 

 

Figure 1. Tanacetum cinerariifolium in ex situ collection of the Institute of Biodiversity and Ecosystem Research, Sofia, Bulgaria (photo: Marina Stanilova).

 

GC/MS analysis

Extraction procedure for GC–MS analysis: A sample of 100 mg of dried plant material as well as internal standards of 50 µL (1 mg/mL) of nonadecanoic acid, ribitol and 3,4 dichloro-4-hidroxy benzoic acid were placed in 2 mL Eppendorf tubes and extracted with 1 mL of MeOH for 24 h at room temperature. Subsequent extract processing was described by Nikolova et al. [2019]. Residue plant material after methanol extraction was hydrolyzed by 2 M NaOH, 4 h, at room temperature.


After acidification to pH 1-2 with conc. HCl, the phenolic compounds were extracted with EtOAc which was dried with anhydrous Na2SO4 and evaporated to obtain methanol insoluble-bound phenolic acids. Phenolic acids of the species were characterized in their three forms: free (identified in methanolic extract), esterified (identified in methanolic extract after alkaline hydrolysis) and methanol insoluble-bound (identified after alkaline hydrolysis in residue material after methanol extraction). Before GC/MS analysis studied, extracts and fractions were dissolved in 50 μL of pyridine and 50 μL of derivatization reagent N,O-bis-(trimethylsilyl)trifluoroacetamide (BSTFA) was added. The samples were heated at 70⁰C for 2 h. After the cooling, the samples were diluted with 300 μL of chloroform and that prepared were analyzed. The GC–MS spectra were recorded on a Termo Scientific Focus GC coupled with Termo Scientific DSQ mass detector operating in EI mode at 70 eV. The conditions of the analysis were presented by Berkov et al. [2021]. The measured mass spectra were deconvoluted using AMDIS 2.64 software before comparison with the databases. Retention Indices (RI) of the compounds were measured with a standard n-alkane hydrocarbons calibration mixture (C9–C36) (Restek, Cat No. 31614, supplied by Teknokroma, Spain).


The compounds were identified by comparing their mass spectra and retention indices (RI) with those of authentic standards and the National Institute of Standards and Technology (NIST) spectra library. The response ratios were calculated for each metabolite relative to the internal standard using the calculated areas for both components.

 

Free radical scavenging activity

The effect of methanolic extracts on DPPH radicals was estimated according to Stanojević et al. [2009]. The results were calculated by Software Prizm 3.00. All experiments were carried out in triplicate.

 

Inhibitory activity on seed germination

A hundred seeds of Lolium perenne were placed in Petri dishes on filter papers moistened with the tested solutions. Methanolic extracts were applied as aqueous solutions at concentration of 1, 2 and 3 mg/mL. Seeds placed in Petri dishes on filter paper moistened with tap water were used as a control. The samples thus prepared were incubated at room temperature for 7 days. At the end of the week the rate of germination inhibition [%] was calculated by Atak et al. [2016]. The reduction in root growth caused by the extracts was estimated as a percentage of the length of the control roots using a formula after cited above source.

 

Acetylcholinesterase (AchEinhibition assay

Acetylcholinesterase inhibitory activity of the methanolic extracts of the species was determined using Ellman's colorimetric method as modified by Lopez et al. [2009]. Extracts from the studied plant parts with concentrations of 0.001, 0.01, 0.01, 0.1 and 1 mg/mL were tested. Galanthamine was used as a positive control.

 

Statistical analysis

Statistical analyses were performed using Microsoft Excel software. The results are presented as mean with standard deviation (SD). The AChE inhibitory and free radical scavenging data were analyzed except Microsoft Excel and the software package Prism 9 (Graph Pad Inc., San Diego, USA). The IC50 values were measured in triplicate and the results are presented as means.

 

RESULTS

Acetone exudates and methanolic extracts of the leaves and the flower heads of Tcinerarifolim were separately analyzed by GC/MS. The results are presented at Table 1.

 

 

Table 1. Compounds identified in methanolic extracts and acetone exudates from leaves and flower heads of Tanacetum cinerariifolim. The values represent peak area ratios of each metabolite relative to the internal standard.

 

 

Compounds

 

RT

 

RI

 

Methanolic extracts

Acetone

exudates

Flower heads

Leaves

Flower heads

Leaves

Pyrethrins

Cinerin I (1)

17.77

2210

53.7

 

 

 

Jasmolin I (2)

19.03

2287

41.2

5.5

 

 

Pyrethrin I (3)

19.18

2296

100.7

24.9

 

 

Cinerin II (4)

23.32

2560

23.5

 

 

 

Jasmolin II (5)

24.46

2636

16.6

 

 

 

Pyrethrin II (6)

24.60

2646

26.7

 

 

 

Fatty acids

Octanoic acid  (7)

8.10

1581

10.7

 

 

 

Hexadecanoic acid C16:0 (Palmitic acid) (8)

13.04

1925

1077.9

686.5

74.3

42.3

Octadecadienoic acid C18:2 (Linoleic acid) (9)

15.83

2090

698.1

386.8

 

 

Octadecenoic acid C18:1 (Oleic acid) (Е) (10)

15.91

2102

12.1

 

 

 

Octadecatrienoic acid C18:3 (Linolenic acid) (11)

15.98

2120

73.9

498.1

 

 

Octadecanoic acid C18:0 (Stearic acid) (12)

16.37

2132

246.9

296.1

 

 

Octadecanoic acid 3-hydroxy (13)

19.39

2309

20.6

 

 

 

Eicosanoic acid C20:0 (Arachidic acid) (14)

25.83

2342

12.8

19.5

 

 

Tricosanoic acid 2-methyl ether(15)

26.82

2710

18.2

4.3

 

 

Tetracosanoic acid C24:0 (Lignoceric acid) (16)

26.89

2740

12.3

26.0

 

 

2-Hydroxytetracosanoic acid (17)

28.17

2801

18.2

2.0

 

 

2-Hydroxypentacosanoic acid (18)

29.49

2914

8.7

1.3

 

 

Fatty alcohols

Dodecanol (19)

7.89

1560

2648.9

169.5

 

 

Tetradecanol (20)

10.46

1759

25.8

8.6

 

 

Octadec-9Z-enol (21)

16.31

2124

29.3

 

 

 

Octadecanol (22)

16.74

2154

47.3

 

12.2

 

Tetracosanol (23)

25.94

2740

72.5

 

108.3

36.5

Hexacosanol (24)

28.66

2935

17.5

 

 

9.8

Sterols and Triterpenes

Campesterol (25)

32.68

3239

161.8

17.7

7.7

 

Stigmasterol (26)

33.04

3319

199.0

67.9

 

 

β-Sitosterol (27)

34.02

3386

972.7

312.6

90.0

 

β-Amyrin (28)

34.34

3415

441.4

24.4

63.2

25.9

Oleanolic acid (29)

35.16

 

 

 

 

78.5

Flavonoids

Scutellarein 6-methyl ether (30)

32.13

3189

 

 

 

9.5

Quercetagetin 3,6,3'-trimethyl ether (31)

33.36

3292

 

 

0.6

196.5

Quercetagetin 3,6-dimethyl ether (32)

33.63

3351

 

 

 

4.9

6-Hydroxyluteolin 6-methyl ether (33)

34.21

3414

 

 

 

31.3

Organic acids

Succinic acid (34)

5.47

1310

295.0

9.8

6.4

37.2

Glyceric acid (35)

5.54

1340

198.3

 

 

53.8

Fumaric acid (36)

5.82

1347

84.6

 

1.7

13.6

Malic acid (37)

6.96

1488

440.1

 

49.4

69.1

Pyroglutamic acid(38)

7.47

1512

331.1

 

5.7

 

Threonic acid (40)

7.52

1545

106.2

 

 

 

Shcimic acid (39)

11.03

1798

 

 

 

142.7

 

Table 1. Continued.

Compounds

 

RT

 

RI

 

Methanolic

extracts

Acetone

exudates

Flower heads

Leaves

Flower heads

Leaves

Sugar and sugar derivatives

meso-Erythritol (41)

7.15

1711

38.7

4.7

 

 

Fructose 1(42)

11.14

1800

242.9

135.0

 

 

Fructose 2 (43)

11.21

1837

1124.7

256.9

51.3

29.6

Glucose (44)

12.40

1882

114.9

 

 

 

Galactose (45)

13.02

1920

191.6

 

 

 

myo-Inositol (46)

13.43

2090

5504.4

195.3

 

 

Sucrose (47)

24.41

2628

3911.5

441.2

396.4

1828.9

Free phenolic acids

Salicylic acid (48)

7.31

1512

42.8

58.9

 

 

4(p)-Hydroxybenzoic acid (49)

8.74

1635

 

16.1

2.6

 

Vanilic acids (50)

10.52

1776

 

 

6.5

18.4

Protocatechuic acid (51)

11.24

1813

 

48.7

39.7

147.1

Quinic acid (52)

11.85

1843

3033.6

5320.5

21.4

2613.9

Caffeic acid trans(53)

16.44

2141

 

157.3

 

 

Chlorgenic acid (54)

30.90

3100

714.7

3271.2

 

250.0

Methanol extractable hydrolysable phenolic acids

Salicylic acid (55)

7.31

1512

5.9

2.4

 

 

3,5-Dihydroxybenzoic acid (56)

9.88

1720

6.2

4.1

 

 

Vanilic acid (57)

10.52

1776

9.7

18.2

 

 

Protocatechuic acid (58)

11.24

1813

44.6

39.3

 

 

Quinic acid (59)

11.85

1843

25.4

58.4

 

 

4(p)- Hydroxycinnamic acid trans (60)

13.27

1948

9.4

12.7

 

 

3,5-Dihydroxybenzoic acid (61)

14.40

2008

60.9

 

 

 

Caffeic acid (62)

16.44

2141

385.1

288.8

 

 

Methanol insoluble hydrolysable bound phenolic acids

4(p)-Hydroxybenzoic acid (63)

8.74

1635

18.1

6.6

 

 

Vanilic acid (64)

10.52

1776

33.6

2.7

 

 

Protocatechuic acid (65)

11.24

1813

210.0

 

 

 

Quinic acid (66)

11.85

1843

103.3

14.0

 

 

4(p)- Hydroxycinnamic acid (67)

13.27

1948

22.4

5.9

 

 

Caffeic acid  cis (68)

13.78

1984

286.8

 

 

 

3,5-Dihydroxybenzoic acid (69)

14.40

2008

3.7

 

 

 

Ferulic acid trans (70)

15.76

2100

12.9

 

 

 

Caffeic acid trans (71)

16.44

2141

3557.2

794.9

 

 

 

 

 

Figure 2. Structures of the identified flavonoid aglycones.

 

Metabolite profiles of acetone exudates

Flavonoid aglycones (30-33), sterols (25,27), fatty acids (8) and alcohols (22-24), organic acids (34-39), free phenolic acids (49-52,54) were found in the acetone exudates. Methyl derivatives of quercetin, apigenin and luteolin, substituted at 6-position were identified as surface flavonoids. Quercetagein 3,6,3-trimethyl ether (jaceidin, 31)  was found as the main flavonoid of both exudates, moreover, it is the only flavonoid identified in the exudate from the flower heads. The rest flavonoid aglycones: scutellarein 6-methyl ether (30), 6-hydroxyluteolin 6-methyl ether (nepetin, 33) and quercetagetin 3,6-dimethyl ether (axillarin, 32) were identified in the exudate of the leaves. The structures of the identified flavonoid aglycones were presented at Figure 2. Campesterol (25), stigmasterol (26) and ß-amayrin (28) were determined as major components in flower heads exudate. Free phenolic acids – chlorogenic (54), quinic (52) and protocatechuic (51) – were found to be major in leaf exudate.

 

Metabolite profiles of metanolic extracts

Pyrethrins (1-6), phenolic (48-71), fatty (7-18) and organic (34-40) acids, sterols (25-27), sugar alcohols (41,46) were identified in the methanolic extracts of flower heads and leaves by GC/MS. The comparative analysis showed that fatty acids and alcohols were mainly concentrated in the flowers and less in the leaves. Palmitic (8), stearic (12) and linoleic (9) acids were established as dominant. Sterols (25-27) and β-amyrin (28) were also proven in flower and leaf extracts, but in flower heads they were in much larger amounts. Saccharides and sugar alcohol (46) were established again in higher quantities in the flower heads extract. Phenolic acids were determined in their free (48,49,51-54), esterified (55-62) and methanol insoluble bound forms (63-71). Free phenolic acids were concentrated mainly in the leaves; chlorogenic acid was determined as major. Alkaline hydrolysable esterified (55-62) and especially bound phenolic acids (63-71) were found in greater amounts in the flower heads extracts than in the leaf extracts. Caffeic acid (71) was determined as the predominant bound phenolic acid. Moreover, this acid was found in high levels in free (53), esterified (62) and methanol insoluble (68,71) forms in both plant organs. Most phenolic acids were found in bound form.

 

Free radical scavenging activity

Methanolic extracts from leaves and flower heads of Tcinerariifolium were evaluated for antiradical activity by DPPH assay. The studied extracts revealed significant activity with IC50=24,49 µg/mL and IC50=45,34 µg/mL for the extracts from the leaves and the flower heads, respectively (Figure 3). However, the leaf extract showed higher activity, due to the higher content of phenolic acids, especially chlorogenic acid (54).

 

 

Figure 3. Free radical scavenging activity of studied Tcinerariifolium extracts against DPPH radicals.

 

Inhibitory activity on seed germination

The inhibitory effect of the studied methanolic extracts on the germination of Lolium perenne seeds was assessed (Table 2).

 

Table 2. Inhibition on seed germination and seed root growth in Lolium perenne under the influence of the studied Tcinerariifolium extracts.

Concentraction (mg/mL)

 

Inhibition of germination (%)

Inhibition of root growth (%)

Extract of leaves

 

Extract of flower heads

Extract of leaves

Extract of flower heads

1

5 ± 3

7 ± 4

17 ± 5

15 ± 4

2

16 ± 5

19 ± 8

45 ± 8

30 ± 6

3

30 ± 9

42 ± 10

66 ± 10

58 ± 9

 

The results showed that the tested extracts exhibited moderate activity in the studied concentration range 1-3 mg/mL. No difference was found in the activity of the extracts from the leaves and from the flower heads. However, more significant inhibition of root growth was established. The percentage of growth inhibition of Lperenne roots was around 50% at the highest concentration tested.

 

Acetylcholinesterase inhibitory activity

The ability of the studied extracts to inhibit acetylcholinesterase was evaluated. In the tested concentration range 0.1-1000 µg/mL a low inhibitory effect (IC50 >1mg/mL) was observed for both extracts.

 

DISCUSSION

Metabolite profiles

Phytochemical profiles of acetone exudates and methanolic extracts from the leaves and flower heads of Tcinerariifolium were determined. Acetone exudates have been widely used in the study of externally located flavonoid aglycones, known also as exudate, surface, and externally flavonoids [Wollenweber and Dietz, 1981; Onyilagha and Grotewold, 2004]. Derivatives of quercetin, apigenin and luteolin 6-O-substituted were identified in the leaf exudate. Flavonoid aglycone – quercetagein 3,6,3'-trimethyl ether (31) has been previously reported as component of flower heads of Tcinerariifolium [Sashida et al., 1983; Kumar et al., 2005]. To the best of our knowledge, flavonoid aglycones: scutellarein 6-methyl ether (30), 6-hydroxyluteolin 6-methyl ether (33) and quercetagetin 3,6-dimethyl ether (32) are reported for the first time here as a component of Tcinerariifolium leaves. These flavonoid aglycones have been reported for other Tanacetum species such as Tanacetum parthenium (L.) Schultz Bip. [Williams et al., 1999; Long et al., 2003; Végh et al., 2018]. Variety of fatty acids as well as sterols was found in the flower head extract, which is in accordance with the data reported by Kumar et al. [2005].

 

In the present study phenolic acids were identified in their free (48-54), esterified (55-62) and methanol insoluble bound (63-71) forms. Chlorogenic acid was found as major free phenolic acid in the leaves. Gecibesler et al. [2016] reported such a result for Tanacetum cilicicum (Boiss.)Grierson. The result obtained that bound phenolic acids predominate in the flower heads suggests that waste products after extraction of pyrethrins can be used for a source of phenolic acids.

 

Biological activity

Tcinerariifolium extracts from flower heads and leaves were evaluated for free radical scavenging, and for inhibition of acetylcholinesterase and seed germination. The antiradical activity of the tested extracts expressed by IC50 values is classified as very active according to the scale described by Reynertson, et al. [2005]. Concerning acetylcholinesterase activity the values obtained (IC50 > 1mg/mL) show low inhibition. Similar results have been reported for extracts and essential oils from other Tanacetum species [Polatoğlu et al., 2015a; Yur et al., 2017]. Moreover, strong AChE activity has been found for essential oils only when applied in pure form, if applied diluted (1-10 mg/mL) their activity is greatly reduced [Polatoğlu et al., 2015b]. Sashida et al. [1983] noted that extract from the flower heads of Ccinerariaefolium inhibited the growth of Chinese cabbage roots, but no more detailed information was found. Comparing the present results with data reported for other species [Wahyuni et al., 2017] the observed inhibition of seed germination can be determined as moderate. Although observed differences in the metabolic profiles of extracts from leaves and flower heads, significant differences in their biological activity was not established. Only the leaf extracts showed slightly higher antioxidant activity than those of the flower heads, which correlates with a higher phenolic acid content in them.

 

CONCLUSION

GC/MS-based analysis on the phytochemical profiles of Tanacetum cinerariifolium leaves and flower heads was conducted. Similar metabolic profiles were found in extracts from different organs, but the leaves had a higher content of free phenolic acids and flavonoid aglycones, while pyrethrins, sterols, fatty acids, sugars, bound phenolic acids were found in larger amounts in the flower heads. Considerable free radical scavenging activity, moderate inhibition on seed germination and low acetylcholinesterase inhibitory activity were established for the studied extracts. Differences between extracts from leaves and flower heads concerning the studied activities were not found. The presented data show that the species is rich in bioactive compounds (especially phenolic acids) that can be isolated from waste plant material after pyrethrin extraction.

 

ACKNOWLEDGEMENTS

This research was supported by the Bulgarian National Science Fund, Bulgarian Ministry of Education and Science (Grant DN 16/2, 11.12.2017).

 

 

AUTHOR CONTRIBUTIONS

All authors contributed to the work presented here, read and approved the final manuscript. MN conceived, designed the experiments, contributed to the GC/MS and antiradical analysis and wrote first version of the manuscript; ETs. carried out the seed germination experiment; VI and MS contributed to collection of the plant material; SB contributed to the AChE and GC/MS analysis and to the writing of the manuscript.

 

CONFLICT OF INTEREST

The authors declare that they hold no competing interests.

 

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OPEN access freely available online

Chiang Mai University Journal of Natural Sciences [ISSN 16851994]

Chiang Mai University, Thailand

https://cmuj.cmu.ac.th

 Milena Nikolova *, Vladimir Ilinkin, Elina Yankova-Tsvetkova, Marina Stanilova and Strahil Berkov

 

Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia, Bulgaria

 

Corresponding author: Milena Nikolova, E-mail: mtihomirova@gmail.com


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Editor: Korakot Nganvongpanit,

Chiang Mai University, Thailand

 

Article history:

Received: January 10, 2022;

Revised: March 16, 2022;

Accepted: March 17, 2022;

Published online: March 24, 2022